Catalytic Reaction of Basidiomycete Lentinula edodes Cytochrome P450, Le.CYP1 Enzyme Produced in Yeast.
نویسندگان
چکیده
The basidiomycete Lentinula edodes (Le.) cytochrome P450, Le.CYP1 was functionally expressed in Saccharomyces cerevisiae. The microsomal fraction containing Le.CYP1 was prepared from the recombinant yeast and the Le.CYP1 was analyzed. The 7-ethoxycoumarin and benzo(a)pyrene were found to be the substrates of Le.CYP1 enzyme. Le.CYP1 converted 7-ethoxycoumarin to 7-hydroxycoumarin.
منابع مشابه
Isolation of genes specifically expressed in the fruit body of the edible basidiomycete Lentinula edodes.
In order to isolate the genes expressed specifically and abundantly in the mature fruit body of Lentinula edodes, the cDNAs derived from the gill of the fruit body were compared with the cDNAs from the mycelia by differential screening. Consequently, six clones were identified as fruit-body-specific genes (fbg03, 08, 13, 14, 16, and 21). The deduced amino acid sequence of fbg14 (Le.cypfb) had s...
متن کاملCloning and characterization of the xyn11A gene from Lentinula edodes.
Hemicellulose represents a rich source of biomass that can be converted into useful chemical feedstocks. One of the main components of hemicellulose is xylan, a polymer of xylose residues. Xylanase enzymes that hydrolyze xylan are therefore of great commercial interest. We have cloned a gene (xyn11A) that encodes a 283-amino acid xylanase enzyme from the fungus Lentinula edodes. The enzyme has ...
متن کاملLentinula edodes produces a multicomponent protein complex containing manganese (II)-dependent peroxidase, laccase and beta-glucosidase.
A multicomponent protein complex containing manganese (II)-dependent peroxidase, laccase and beta-glucosidase was isolated from culture extracts of the white rot basidiomycete Lentinula edodes. This protein complex showed a single protein band on native polyacrylamide gel electrophoresis (PAGE). On sodium dodecyl sulfate (SDS)-PAGE, however, it displayed three major bands and several additional...
متن کاملLong terminal repeat of the mushroom retrotransposon functions as a dual terminator to transcription.
The 474-bp long terminal repeat (LTR) of the basidiomycete Lentinula edodes retrotransposon was found to contain a different type of transcription termination-poly (A)-addition signal on each strand: TATGT- - -TATG- - -TCT, first reported in yeast genes, and an AATAA (or ACATAA)- - -GT(Py)-rich sequence, frequently found in mammalian genes.
متن کاملThe Extracellular Indolic Compounds of Lentinus edodes
In macrobasidial fungi, the properties of compounds of a phytohormonal nature, which are well known in higher plants and are intensively studied in soil associative microorganisms, are only described in an unsystematic manner and, apparently, to an insufficient degree. Auxins are the most studied group of phytohormonal substances. As an object of research, along with other mycological objects o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Bioscience, biotechnology, and biochemistry
دوره 68 1 شماره
صفحات -
تاریخ انتشار 2004